This is the recipe for the crystal violet stain used in the Gram stain and other staining procedures.
Crystal Violet Ingredients
- 2 g crystal violet
- 20 ml 95% ethyl alcohol
- 0.8 g ammonium citrate monohydrate
- 80 ml deionized water
Prepare Crystal Violet Stain
- Dissolve 2 g crystal violet in 20 ml of 95% ethyl alcohol.
- Dissolve 0.8 g ammonium oxalate monohydrate in 80 ml deionized water.
- Mix the crystal violet and ammonium oxalate monohydrate solutions to make the crystal violet stain. Filter the stain if necessary.
