1. Education

Discuss in my forum

Crystal Violet Stain

Recipe for Crystal Violet for the Gram Stain


This is the recipe for the crystal violet stain used in the Gram stain and other staining procedures.

Crystal Violet Ingredients

  • 2 g crystal violet
  • 20 ml 95% ethyl alcohol
  • 0.8 g ammonium citrate monohydrate
  • 80 ml deionized water

Prepare Crystal Violet Stain

  1. Dissolve 2 g crystal violet in 20 ml of 95% ethyl alcohol.

  2. Dissolve 0.8 g ammonium oxalate monohydrate in 80 ml deionized water.

  3. Mix the crystal violet and ammonium oxalate monohydrate solutions to make the crystal violet stain. Filter the stain if necessary.
  1. About.com
  2. Education
  3. Chemistry
  4. Chemistry Lab
  5. Lab Recipes
  6. Crystal Violet Stain - Crystal Violet for the Gram Stain

©2014 About.com. All rights reserved.