1. Education
Send to a Friend via Email
You can opt-out at any time. Please refer to our privacy policy for contact information.

Discuss in my forum

Recipe for Crystal Violet for the Gram Stain

Recipe for Crystal Violet for the Gram Stain


This is the recipe for the crystal violet stain used in the Gram stain and other staining procedures.

Crystal Violet Ingredients

  • 2 g crystal violet
  • 20 ml 95% ethyl alcohol
  • 0.8 g ammonium citrate monohydrate
  • 80 ml deionized water

Prepare Crystal Violet Stain

  1. Dissolve 2 g crystal violet in 20 ml of 95% ethyl alcohol.


  2. Dissolve 0.8 g ammonium oxalate monohydrate in 80 ml deionized water.


  3. Mix the crystal violet and ammonium oxalate monohydrate solutions to make the crystal violet stain. Filter the stain if necessary.

©2014 About.com. All rights reserved.