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10X TBE Electrophoresis Buffer

Recipe for TBE Buffer

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A buffer resists changes in pH.

A buffer resists changes in pH.

Cl4ss1cr0ck3R, Creative Commons
This is the protocol or recipe for preparing 10X TBE electrophoresis buffer:

10X TBE Electrophoresis Buffer Materials

  • 108 g of Tris base [tris(hydroxymethyl)aminomethane]
  • 55 g of boric acid
  • 7.5 g of EDTA, disodium salt
  • deionized water

Prepare the 10X TBE Electrophoresis Buffer

  1. Dissolve the Tris, boric acid and EDTA in 800 ml of deionized water.

     

  2. Dilute the buffer to 1 L. Undissolved white clumps may be made to dissolve by placing the bottle of solution in a hot water bath. You do not need to sterilize the solution.

10X TBE Electrophoresis Buffer Storage

Store the bottle of 10X buffer solution at room temperature.

Using 10X TBE Electrophoresis Buffer

The solution is diluted before use. Dilute 100 mL of 10X stock to 1 L with deionized water.

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