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Vitamin C Determination by Iodine Titration

By Anne Marie Helmenstine, Ph.D., About.com

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How to Calculate Vitamin C

Titration Calculations

  1. Calculate the ml of titrant used for each flask. Take the measurements you obtained and average them.

    average volume = total volume / number of trials

  2. Determine how much titrant was required for your standard.

    If you needed an average of 10.00 ml of iodine solution to react 0.250 grams of vitamin C, then you can determine how much vitamin C was in a sample. For example, if you needed 6.00 ml to react your juice (a made-up value - don't worry if you get something totally different):

    10.00 ml iodine solution / 0.250 g Vit C = 6.00 ml iodine solution / X ml Vit C

    40.00 X = 6.00

    X = 0.15 g Vit C in that sample

  3. Keep in mind the volume of your sample, so you can make other calculations, such as grams per liter. For a 25 ml juice sample, for example:

    0.15 g / 25 ml = 0.15 g / 0.025 L = 6.00 g/L of vitamin C in that sample

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