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Vitamin C Determination by Iodine Titration


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Procedure for Determining Vitamin C
Orange juice is an excellent source of Vitamin C.

Orange juice is an excellent source of Vitamin C.

Andrew Unangst, Getty Images


The goal of this laboratory exercise is to determine the amount of vitamin C in samples, such as fruit juice.


The first step is to prepare the solutions. I've listed examples of quantities, but they aren't important. What matters is that you know the concentration of the solutions and the volumes that you use.

Preparing Solutions

1% Starch Indicator Solution

  1. Add 0.50 g soluble starch to 50 near-boiling distilled water.
  2. Mix well and allow to cool before use. (doesn't have to be 1%; 0.5% is fine)

Iodine Solution

  1. Dissolve 5.00 g potassium iodide (KI) and 0.268 g potassium iodate (KIO3) in 200 ml of distilled water.
  2. Add 30 ml of 3 M sulfuric acid.
  3. Pour this solution into a 500 ml graduted cylinder and dilute it to a final volume of 500 ml with distilled water.
  4. Mix the solution.
  5. Transfer the solution to a 600 ml beaker. Label the beaker as your iodine solution.

Vitamin C Standard Solution

  1. Dissolve 0.250 g vitamin C (ascorbic acid) in 100 ml distilled water.
  2. Dilute to 250 ml with distilled water in a volumetric flask. Label the flask as your vitamin C standard solution.

Standardizing Solutions

  1. Add 25.00 ml of vitamin C standard solution to a 125 ml Erlenmeyer flask.
  2. Add 10 drops of 1% starch solution.
  3. Rinse your buret with a small volume of the iodine solution and then fill it. Record the initial volume.
  4. Titrate the solution until the endpoint is reached. This will be when you see the first sign of blue color that persists after 20 seconds of swirling the solution.
  5. Record the final volume of iodine solution. The volume that was required is the starting volume minus the final volume.
  6. Repeat the titration at least twice more. The results should agree within 0.1 ml.
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